Document Type : Original Article

Author

• Behzad Kaviani

Islamic Azad University, Rahst Branch

Abstract

The most suitable propagation method of Petunia is in vitro propagation. The present study reports the results of three experiments. These three experiments include investigating the effect of different concentrations of plant growth regulators; 6-benzylaminopurine (BA) (0, 0.4, 0.8 and 1.2 mg/l) and 2,4-D (0, 0.5, 1 and 1.5 mg/l), using solid culture medium and two-phase culture system (solid or semi-solid and liquid culture medium) and using iron chelate Nano-fertilizer (0.05, 0.075, 0.1, 0.125 and 0.15 mg/l) were on micropropagation of Petunia Hybrid (Petunia × hybrida L. cv. ‘Opera’). Lateral buds cut from young seedlings grown in vitro at 30 days old, germinated from hybrid seeds on Murashige and Skoog (MS) medium, were used as explants. The results showed that the highest number of leaves (27.3) and the highest dry weight of the plant (0.32 g) were obtained in the medium without growth regulators (control). The highest number of nodes (13.3) and the longest root length (6.3 mm) were recorded in the medium enriched with 0.5 and 1.5 mg/l of 2,4-D, respectively. The highest dry weight of callus (0.28 g) was obtained from the application of BA at the concentration of 0.8 mg/l. The highest shoot length (17.16 mm) was recorded in the two-phase culture medium without growth regulators (control). The use of iron chelate Nano-fertilizer did not affect the main and important parameters of the micropropagation of Petunia and did not increase their efficiency.

Keywords

• "Auxins
• " cytokinins
• " ornamental plants
• "

Main Subjects

• Ornamrntal Plants